List Of Some Quantitative identification of Disaccheride, Polysaccheride & Protein

List Of Some Quantitative identification of Disaccheride, Polysaccheride & ProteinTest For Disaccheride :-

1. Hydrolysis Test :-

- Take 2.5 ml of Sample (Sucrose) in Beaker- Add 4-5 ml drop Of Concentrated HCl into it- Boil Solution Mix it Constantly Till Quantity reduced to half (1/2) - Now Divide Solution into 2 Test tube , Then mark both test tube 'A' & 'B' for Fehling & Benedict Test respectively 

2. Fearjen's Test :-

Colour Test For Polysaccheride :-

1. Iodine Test For Starch :-

- Take 2 ml of Test solution (starch) in Test Tube - Boil & cool it at Room temperature - Then add 2-3 ml of iodine solution

Observation :-

Blue Colour Appear 

Result :-

Presence Of starch is Confirmed

2. Iodine Test For Glycogen :-

- Take 2 ml of Test solution in Test Tube - Boil & cool it at Room temperature - Then add 2-3 ml of iodine solution

Observation :-

Colour Disappear on heating

Result :-

Presence Of glycogen is Confirmed

Test For Albumin (protein)

1. Biurate test :-

- take 3 ml of test Solution in test tube (protein = Albumin) - Add 1 ml of 40 % NaOH- Add 2 drop of 1% of CuSO4

Observation :-

Violet & Pink Colour , Which may dissappear on Adding excess of CuSO4

Result :-

Presence Of protein (albumin) is Confirmed

2. Xanthoproteic Test :-

- Take 2 ml of test solution - Add 0.5 ml of HNO3 into it- Slowly heat it Boil & Cool it under Tap water- Add 40% NaOH to make it Alkaline

Observation :-

Yellow & Turn Orange On Addition Of NaOH

Result :-

Presence Of albumin Is Confirmed

3. Mercury Chloride Test :-

- Take 2 ml of test solution - Add Mercury Chloride Into It 

Observation :- 

white ppt formed

Result :-

Presence Of albumin Is Confirmed

@ Reducing & Non-reducing Test :-

1. Methylene Blue Test :-

- take 3 ml of Distilled water - Add 1 Drop of methylene Blue - Add 1 ml of test solution- Then 1 ml of 40% NaOH

Observation :-

Blue colour Dissappear 

Result :-

Test Solution Contain Reducing Sugar

2. Picric Acid Test :-

- Take 3ml of Test Solution - Add 1 ml of picric Acid Solution into it- Add 1 ml of 40% NaOH solution

Observation :-

Red Colour Appear

Result :-

Test Solution Contain Reducing Sugar

3. Tommer's Test :-

- Take 2ml of 0.5 ml of CuSO4 - Add 2 ml of Test Solution - Add 2 ml of 40% Of NaOH

Observation :-

Yellow Or Brick red Colour

Result :-

Test solution Contain Reducing Sugar