Quantitative identification of Simple Sugar

List Of Some Quantitative identification of Simple Sugar (monosaccheride)

@ General Test :-
1. Mollisch test :-
Step are
- Take 2 test tube , mark Then A & B
- Then take 2 to 3 ml of Given sample (sugar) in 'A' test tube
- Then Add 2 drop of mollisch Reagent into it
- Shake test tube 'A' Very well
- Take 3-4 ml Concentrated H2SO4 in test tube 'B' ,
- Then with care , gently add H2SO4 in test tube 'A' from 'B'
Observation :-
While adding H2SO4 into Test tube 'B' , A pink or violet colour ring appear at junction of both while mixing
Inferance :-
given sample contain carbohydrates

@ Test For Fructose :-
2. Faugler's Test :-
Step are
- Take about 5 ml ogf given sample (fructose) in test tube &
- Add 3 ml of faugler sample into it
- Boil & Shake test Tube Gently
Observation :-
Blue colour appear
Inferance :-
Given sample contain Fructose

3. Sawniroff test :-
- Take 3ml of sawrinoff agent
- Add 1ml of test solution
- Boil it for 30 Sec
Observation :-
Red to orange colour appear
Inferance :-
Presence Of fructose is Confirmed

@ Test For Glucose :-
1. Benedict Test :-
- Take 5 ml of benedict Reagnent in Test Tube
- Add .5ml of test solution (glucose) in it
- Mix both solution well & Boil For two minute
- Then cool it under tap water
Observation :-
yellow cream Or Brick red ppt Formed
Inferance :-
Test Solution Contain Reducing Monosaccheride (glucose)

2. Fehling Test :-
- Take 3 ml of 1% potassium ferring cyanide in Test Tube
- Add 1 ml of NaOH solution into it
- Boil & Then Add Near 10 drop of test solution (glucose) & keep it to cool
Observation :-
Yellow colour ppt. of ferricyanide become to fade & finally decolourized
Brick red to Colorless
Inferance :-
Test solution Contain Reducing Ferricyanide